Molecular Cloning of Leishmania major gp63 Gene in BALB/c Mouse CT26 Cell Line

CLONING AND EXPRESSION OF LEISHMANOLYSIN GENE FROM LEISHMANIA MAJOR IN PRIMATE CELL LINES

Leishmanolysin is a worldwide disease that is caused by different species of the genus Leishmania. Leishmanolysin, One of the genes expressed by Leishmania, appears to be an ideal candidate for genetic vaccination. In this study, a full length sequence, which encodes Leishmanolysin functionally critical regions (amino acids 100-579), was cloned from a Leishmania strain endemic to Iran. Analysis...

Expression of Gp63 Gene from NIH Strain of Leishmania major in Pichia pastoris

Leishmaniasis is a major infectious disease of considerable public health in more than 86 countries around the world. Several approaches toward vaccine development against this disease have been taken. Glycoprotein (gp63) is conserved among diverse species of Leishmania and has induced immunological responses in murine models. Therefore, this glycoprotein has been considered as a second generat...

Study of Leishmania Tropica proliferation in mouse J774A-1 cell line.

cloning and expression of leishmanolysin gene from leishmania major in primate cell lines

leishmanolysin is a worldwide disease that is caused by different species of the genus leishmania. leishmanolysin, one of the genes expressed by leishmania, appears to be an ideal candidate for genetic vaccination. in this study, a full length sequence, which encodes leishmanolysin functionally critical regions (amino acids 100-579), was cloned from a leishmania strain endemic to iran. analysis...

Molecular cloning, expression and characterization of ribokinase of Leishmania major.

Ribokinase (EC 2.1.7.15) from Leishmania major was cloned, sequenced and overexpressed in Escherichia coli. The gene expressed an active enzyme that had comparable activity to the same enzyme studied in E. coli. It specifically phosphorylated D-ribose. Under defined conditions, the K(m) for the substrates D-ribose and ATP were 0.3+/-0.04 mM and 0.2+/-0.02 mM, respectively. The turnover numbers ...